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antibody against mac2  (Cedarlane)


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    Cedarlane antibody against mac2
    Antibody Against Mac2, supplied by Cedarlane, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+mac2/Anti-Mouse%2FHuman+Mac-2+(Galectin-3)%2C+Purified+(Clone+M3%2F38)+(rat+IgG2a)/pm37629170-260-7-10
    Average 96 stars, based on 398 article reviews
    antibody against mac2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Avidin-Biotin Assay:

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia
    Article Snippet: .. After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98°C for 10 min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4°C overnight. .. Sections were then incubated with a biotinylated rabbit anti-rat IgG secondary antibody (1:200, BA-4001-0.5, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature, followed by incubation with ABC solution (PK-6100, Vector Laboratories, Burlingame, CA, USA) for 30 min at room temperature.

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia.
    Article Snippet: .. After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98◦C for 10min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4◦C overnight. .. Sections were then incubated with a biotinylated rabbit anti-rat IgG secondary antibody (1:200, BA-4001-0.5, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature, followed by incubation with ABC solution (PK-6100, Vector Laboratories, Burlingame, CA, USA) for 30min at room temperature.

    Incubation:

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia
    Article Snippet: .. After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98°C for 10 min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4°C overnight. .. Sections were then incubated with a biotinylated rabbit anti-rat IgG secondary antibody (1:200, BA-4001-0.5, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature, followed by incubation with ABC solution (PK-6100, Vector Laboratories, Burlingame, CA, USA) for 30 min at room temperature.

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia.
    Article Snippet: .. After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98◦C for 10min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4◦C overnight. .. Sections were then incubated with a biotinylated rabbit anti-rat IgG secondary antibody (1:200, BA-4001-0.5, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature, followed by incubation with ABC solution (PK-6100, Vector Laboratories, Burlingame, CA, USA) for 30min at room temperature.

    Article Title: Myeloid-Specific Deletion of the AMPKα2 Subunit Alters Monocyte Protein Expression and Atherogenesis
    Article Snippet: Prior to staining, the sections were washed with phosphate buffered saline (PBS) and blocked with 0.3% bovine serum albumin, 5% horse serum (Fisher Scientific) and 0.1% Triton X-100 for 1 h at room temperature. .. The antibodies against Mac2 (CL8942AP, Cedarlane/Biozol, 1:200, Eching, Germany), MMP9 (M9570, Sigma, 1:200) and Collagen IV (1340-01, SouthernBiotech/Biozol, 1:1000, Eching, Germany) were diluted in PBS and incubated at 4°C overnight. .. After washing four times with PBS, the secondary antibodies (1:300, donkey anti-rat AB150155, Abcam, Cambridge, Great Britain), or anti-goat Alexa Fluor 568 (1:300, A-11057, Invitrogen/Thermo Fisher Scientific, Frankfurt, Germany) were incubated with FITC conjugated smooth muscle actin antibody (F377, 1:1000, Sigma-Aldrich/Merck).

    Blocking Assay:

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia
    Article Snippet: .. After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98°C for 10 min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4°C overnight. .. Sections were then incubated with a biotinylated rabbit anti-rat IgG secondary antibody (1:200, BA-4001-0.5, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature, followed by incubation with ABC solution (PK-6100, Vector Laboratories, Burlingame, CA, USA) for 30 min at room temperature.

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia.
    Article Snippet: .. After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98◦C for 10min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4◦C overnight. .. Sections were then incubated with a biotinylated rabbit anti-rat IgG secondary antibody (1:200, BA-4001-0.5, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature, followed by incubation with ABC solution (PK-6100, Vector Laboratories, Burlingame, CA, USA) for 30min at room temperature.

    other:

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: For PHACTR1 staining, sections were incubated with anti-PHACTR1 antibody (Abcam, ab229120; 1:100) overnight at 4°C.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: .. The other thymus lobe was formalin-fixed, paraffin-embedded, and sectioned, followed by staining of the sections (5 mm) with TUNEL reagents (Roche) and antibodies against Mac2 (Cedarlane, CL8942LE; 1:1000), phospho-MLC2 (Cell Signaling Technology, 3671; 1:100), and total MLC2 (Abcam, ab79935; 1:100). .. After incubation in serum-free protein blocking buffer (DAKO, catalog X0909) for 1 hour, sections were incubated overnight at 4°C with primary antibody directed toward CD68 (DAKO, catalog M0814; 1:100) for the in situ efferocytosis assay described below.

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: .. The other thymus lobe was formalin-fixed, paraffin-embedded, and sectioned, followed by staining of the sections (5 mm) with TUNEL reagents (Roche) and antibodies against Mac2 (Cedarlane, CL8942LE; 1:1000), phospho-MLC2 (Cell Signaling Technology, 3671; 1:100), and total MLC2 (Abcam, ab79935; 1:100). ..

    Staining:

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: .. The other thymus lobe was formalin-fixed, paraffin-embedded, and sectioned, followed by staining of the sections (5 mm) with TUNEL reagents (Roche) and antibodies against Mac2 (Cedarlane, CL8942LE; 1:1000), phospho-MLC2 (Cell Signaling Technology, 3671; 1:100), and total MLC2 (Abcam, ab79935; 1:100). .. After incubation in serum-free protein blocking buffer (DAKO, catalog X0909) for 1 hour, sections were incubated overnight at 4°C with primary antibody directed toward CD68 (DAKO, catalog M0814; 1:100) for the in situ efferocytosis assay described below.

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: .. The other thymus lobe was formalin-fixed, paraffin-embedded, and sectioned, followed by staining of the sections (5 mm) with TUNEL reagents (Roche) and antibodies against Mac2 (Cedarlane, CL8942LE; 1:1000), phospho-MLC2 (Cell Signaling Technology, 3671; 1:100), and total MLC2 (Abcam, ab79935; 1:100). ..

    Article Title: Metabolically activated adipose tissue macrophages link obesity to triple-negative breast cancer
    Article Snippet: .. Adipose tissue was sectioned (5 μm) and stained with antibodies against MAC2 (Cedarlane) and PLIN2 (Abcam). .. Fluorescence images were acquired with a Nikon Eclipse Ti2 microscope with the following setting: objective magnification 20, objective numerical aperture 0.45, room temperature, emission wavelength of DAPI (457.5 nm), GFP (535.0 nm), and RFP (610 nm), Camera Nikon DS-Qi2, and NIS-Element Version 5.02 software.

    TUNEL Assay:

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: .. The other thymus lobe was formalin-fixed, paraffin-embedded, and sectioned, followed by staining of the sections (5 mm) with TUNEL reagents (Roche) and antibodies against Mac2 (Cedarlane, CL8942LE; 1:1000), phospho-MLC2 (Cell Signaling Technology, 3671; 1:100), and total MLC2 (Abcam, ab79935; 1:100). .. After incubation in serum-free protein blocking buffer (DAKO, catalog X0909) for 1 hour, sections were incubated overnight at 4°C with primary antibody directed toward CD68 (DAKO, catalog M0814; 1:100) for the in situ efferocytosis assay described below.

    Article Title: Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis
    Article Snippet: .. The other thymus lobe was formalin-fixed, paraffin-embedded, and sectioned, followed by staining of the sections (5 mm) with TUNEL reagents (Roche) and antibodies against Mac2 (Cedarlane, CL8942LE; 1:1000), phospho-MLC2 (Cell Signaling Technology, 3671; 1:100), and total MLC2 (Abcam, ab79935; 1:100). ..

    Immunostaining:

    Article Title: JNK1 ablation improves pancreatic β‐cell mass and function in db/db diabetic mice without affecting insulin sensitivity and adipose tissue inflammation
    Article Snippet: .. The number of adipose tissue crown‐like structures (CLS) was quantified by immunostaining of paraffin embedded sections of adipose tissues from of db/db‐JNK1 −/− mice and db/db control mice using antibodies against Mac2 (Cederlane, #CL8942AP) as previously reported., Images were acquired at a 20× magnification on a Zeiss AxioPlan microscope (AxioVision Software). ..

    Control:

    Article Title: JNK1 ablation improves pancreatic β‐cell mass and function in db/db diabetic mice without affecting insulin sensitivity and adipose tissue inflammation
    Article Snippet: .. The number of adipose tissue crown‐like structures (CLS) was quantified by immunostaining of paraffin embedded sections of adipose tissues from of db/db‐JNK1 −/− mice and db/db control mice using antibodies against Mac2 (Cederlane, #CL8942AP) as previously reported., Images were acquired at a 20× magnification on a Zeiss AxioPlan microscope (AxioVision Software). ..

    Microscopy:

    Article Title: JNK1 ablation improves pancreatic β‐cell mass and function in db/db diabetic mice without affecting insulin sensitivity and adipose tissue inflammation
    Article Snippet: .. The number of adipose tissue crown‐like structures (CLS) was quantified by immunostaining of paraffin embedded sections of adipose tissues from of db/db‐JNK1 −/− mice and db/db control mice using antibodies against Mac2 (Cederlane, #CL8942AP) as previously reported., Images were acquired at a 20× magnification on a Zeiss AxioPlan microscope (AxioVision Software). ..

    Software:

    Article Title: JNK1 ablation improves pancreatic β‐cell mass and function in db/db diabetic mice without affecting insulin sensitivity and adipose tissue inflammation
    Article Snippet: .. The number of adipose tissue crown‐like structures (CLS) was quantified by immunostaining of paraffin embedded sections of adipose tissues from of db/db‐JNK1 −/− mice and db/db control mice using antibodies against Mac2 (Cederlane, #CL8942AP) as previously reported., Images were acquired at a 20× magnification on a Zeiss AxioPlan microscope (AxioVision Software). ..



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    Visceral adipose tissue inflammation. Immune cell subsets were analyzed in visceral adipose tissue (VAT) homogenates of End.LepR-WT and End.LepR-KO mice fed high-fat diet (HFD) for 16 weeks using flow cytometry. Representative dot plots after analysis of MHC-II and Ly6C are shown in ( A ), of CD11c and MHC11 in ( C ), the results of the quantitative analysis of CD45 + Lin − CD11b + Ly6C high CXCR1 + MHCII − cells in ( B ) and of CD45 + Lin − CD11b − CD11c + MHCII − in ( D ), respectively. Data are expressed as % of total living cells in VAT and were compared using One-Way ANOVA, Sidak’s multiple comparisons test. *p < 0.05, **p < 0.01 and ***p < 0.005. ( E ) Representative images after immunohistochemical analysis of macrophages in VAT of End.LepR-WT and End.LepR-KO mice fed HFD for 16 weeks. Scale bars represent 100 μm. The results of the quantitative analysis of the <t>Mac2-immunopositive</t> area are shown in ( F ), the number of crown-like structures (CLS) or nucleated giant cells per 100 adipocytes are shown in ( G ) and ( H ), respectively. Data were analyzed using Student’s t test. *p < 0.05 and **p < 0.01.
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    Image Search Results


    Visceral adipose tissue inflammation. Immune cell subsets were analyzed in visceral adipose tissue (VAT) homogenates of End.LepR-WT and End.LepR-KO mice fed high-fat diet (HFD) for 16 weeks using flow cytometry. Representative dot plots after analysis of MHC-II and Ly6C are shown in ( A ), of CD11c and MHC11 in ( C ), the results of the quantitative analysis of CD45 + Lin − CD11b + Ly6C high CXCR1 + MHCII − cells in ( B ) and of CD45 + Lin − CD11b − CD11c + MHCII − in ( D ), respectively. Data are expressed as % of total living cells in VAT and were compared using One-Way ANOVA, Sidak’s multiple comparisons test. *p < 0.05, **p < 0.01 and ***p < 0.005. ( E ) Representative images after immunohistochemical analysis of macrophages in VAT of End.LepR-WT and End.LepR-KO mice fed HFD for 16 weeks. Scale bars represent 100 μm. The results of the quantitative analysis of the Mac2-immunopositive area are shown in ( F ), the number of crown-like structures (CLS) or nucleated giant cells per 100 adipocytes are shown in ( G ) and ( H ), respectively. Data were analyzed using Student’s t test. *p < 0.05 and **p < 0.01.

    Journal: Scientific Reports

    Article Title: Deletion of endothelial leptin receptors in mice promotes diet-induced obesity

    doi: 10.1038/s41598-023-35281-7

    Figure Lengend Snippet: Visceral adipose tissue inflammation. Immune cell subsets were analyzed in visceral adipose tissue (VAT) homogenates of End.LepR-WT and End.LepR-KO mice fed high-fat diet (HFD) for 16 weeks using flow cytometry. Representative dot plots after analysis of MHC-II and Ly6C are shown in ( A ), of CD11c and MHC11 in ( C ), the results of the quantitative analysis of CD45 + Lin − CD11b + Ly6C high CXCR1 + MHCII − cells in ( B ) and of CD45 + Lin − CD11b − CD11c + MHCII − in ( D ), respectively. Data are expressed as % of total living cells in VAT and were compared using One-Way ANOVA, Sidak’s multiple comparisons test. *p < 0.05, **p < 0.01 and ***p < 0.005. ( E ) Representative images after immunohistochemical analysis of macrophages in VAT of End.LepR-WT and End.LepR-KO mice fed HFD for 16 weeks. Scale bars represent 100 μm. The results of the quantitative analysis of the Mac2-immunopositive area are shown in ( F ), the number of crown-like structures (CLS) or nucleated giant cells per 100 adipocytes are shown in ( G ) and ( H ), respectively. Data were analyzed using Student’s t test. *p < 0.05 and **p < 0.01.

    Article Snippet: Sections were incubated overnight at 4 °C with primary rat monoclonal antibody against Mac2 (CL8942AP; Cedarlane Laboratories; dilution, 1:400 in antibody diluent (Dako)).

    Techniques: Flow Cytometry, Immunohistochemical staining

    Brain inflammation and energetic profiles of primary brain and VAT endothelial cells. Summary of findings after analysis of glycolysis and mitochondrial respiration in living primary endothelial cells isolated from brain (A,B) or visceral adipose tissue (VAT; C , D ) of female End.LepR-WT (n = 4–5) and End.LepR-KO (n = 7) mice fed standard laboratory diet (SD) or high-fat diet (HFD) for 16 weeks. Analyses were performed using the Seahorse XF96e Extracellular Flux analyzer. Results were normalized to the number of cells and are expressed as -fold change vs. End.LepR-WT mice. Data were analyzed using One-Way ANOVA, Sidak’s multiple comparisons test. Ns, non-significant. Representative immunofluorescence images of cryo-embedded brain sections from End.LepR-WT (n = 7–8) and End.LepR-KO (n = 7) mice, fed high-fat diet for 16 weeks, after staining with antibodies against CD206 (green) and CD31 (red) ( E ) or against Mac2 (green) and VEGF (red) ( H ). Size bars represent 20 µm. Results after quantification of the area immunopositive for CD206 ( F ), CD31 ( G ), Mac2 ( I ) or VEGF ( J ), expressed per total area of 40 × microscopic fields. Data were analyzed using Student’s t test. *p < 0.05 and **p < 0.01.

    Journal: Scientific Reports

    Article Title: Deletion of endothelial leptin receptors in mice promotes diet-induced obesity

    doi: 10.1038/s41598-023-35281-7

    Figure Lengend Snippet: Brain inflammation and energetic profiles of primary brain and VAT endothelial cells. Summary of findings after analysis of glycolysis and mitochondrial respiration in living primary endothelial cells isolated from brain (A,B) or visceral adipose tissue (VAT; C , D ) of female End.LepR-WT (n = 4–5) and End.LepR-KO (n = 7) mice fed standard laboratory diet (SD) or high-fat diet (HFD) for 16 weeks. Analyses were performed using the Seahorse XF96e Extracellular Flux analyzer. Results were normalized to the number of cells and are expressed as -fold change vs. End.LepR-WT mice. Data were analyzed using One-Way ANOVA, Sidak’s multiple comparisons test. Ns, non-significant. Representative immunofluorescence images of cryo-embedded brain sections from End.LepR-WT (n = 7–8) and End.LepR-KO (n = 7) mice, fed high-fat diet for 16 weeks, after staining with antibodies against CD206 (green) and CD31 (red) ( E ) or against Mac2 (green) and VEGF (red) ( H ). Size bars represent 20 µm. Results after quantification of the area immunopositive for CD206 ( F ), CD31 ( G ), Mac2 ( I ) or VEGF ( J ), expressed per total area of 40 × microscopic fields. Data were analyzed using Student’s t test. *p < 0.05 and **p < 0.01.

    Article Snippet: Sections were incubated overnight at 4 °C with primary rat monoclonal antibody against Mac2 (CL8942AP; Cedarlane Laboratories; dilution, 1:400 in antibody diluent (Dako)).

    Techniques: Isolation, Immunofluorescence, Staining

    Myeloid-specific Pfkfb3 deficiency attenuates LPS-induced inflammatory responses. (A) Representative images (left) and quantification (right) for immunohistochemical staining of the neutrophil marker Ly6G in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (B) Representative images (left) and quantification (right) for immunohistochemical staining of the macrophage marker Mac2 in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (C–E) qPCR analysis of the mRNA levels of Il1b (C) , Il6 (D) and Nos2 (E) in the lung of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 6). (F,G) ELISA analysis of Il1b (F) and Il6 (G) in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 4). (H) NO levels in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 3–4). All data are represented as mean ± SEM, ** P < 0.01 and *** P < 0.001 for Pfkfb3 WT vs. Pfkfb3 ΔMϕ (unpaired two-tailed Student's t test).

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia

    doi: 10.3389/fcvm.2021.745810

    Figure Lengend Snippet: Myeloid-specific Pfkfb3 deficiency attenuates LPS-induced inflammatory responses. (A) Representative images (left) and quantification (right) for immunohistochemical staining of the neutrophil marker Ly6G in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (B) Representative images (left) and quantification (right) for immunohistochemical staining of the macrophage marker Mac2 in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (C–E) qPCR analysis of the mRNA levels of Il1b (C) , Il6 (D) and Nos2 (E) in the lung of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 6). (F,G) ELISA analysis of Il1b (F) and Il6 (G) in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 4). (H) NO levels in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 3–4). All data are represented as mean ± SEM, ** P < 0.01 and *** P < 0.001 for Pfkfb3 WT vs. Pfkfb3 ΔMϕ (unpaired two-tailed Student's t test).

    Article Snippet: After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98°C for 10 min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4°C overnight.

    Techniques: Immunohistochemical staining, Staining, Marker, Injection, Enzyme-linked Immunosorbent Assay, Two Tailed Test